Day 13- Chromatography: Principle of distribution/partition

Proteomics Research Internship Schedule

Proteomics Research Internship Recorded Sessions

Day 1 : Introduction to Protein as macromolecule and its building blocks. Physico-Chemico-Biological properties of amino acids. (51:46)
DAY-2 Anabolism of Proteins and their regulation. (56:15)
DAY-3 Catabolism of Proteins and their regulation. (60:42)
DAY-4 Disorders and Diseases due to protein dysregulation. (41:25)
DAY-5 Understanding Primary, Secondary (GORV), Tertiary (CPH Model), Quaternary Structures and their intra- and inter-molecular association. (58:43)
DAY-6 In silico Pathway analysis with KEGG and Protein data bank (PDB). (59:50)
DAY-7 Protein-protein interaction network, STRING, Network terminology, and Pathway enrichment analysis. (58:24)
DAY-8 Overview of protein analysis with special reference to upstream and downstream processes. (60:56)
DAY-9 Purpose and tools available for protein Isolation, Elucidation, Identification, Characterization, Nomenclature, and Function. (59:13)
Day -10 Qualitative and Quantitative methods for proteins detection and estimation (60:17)
Day -11 Spectroscopy: Fundamentals of spectra. Detailed Principle, Instrumentation, and protocols (SOPs) for Visible Spectroscopy. Result analysis and interpretation. (60:25)
Day 12 - Detailed Principle, Instrumentation, and protocols (SOPs) for UV-Spectroscopy and Luminometry. Result analysis and interpretation. (62:00)
Day 13- Chromatography: Principle of distribution/partition coefficient. Absorption and Adsorption phenomenon, Retention factor (Rf and Rt concepts). Types: Planar and Columnar (non-pressure and pressure-induced systems). (62:32)
Day 14 - A detailed explanation of Paper, TLC, 2D-TLC, HPTLC, and applications (60:19)
Day 15- Concepts of Column Chromatography. Calculation of Gel swelling, matrix preparation, Bed volume, Void volume, and Theoretical plates. (42:42)
Day 16- Types and applications of Column Chromatography: Gel filtration, Ion exchange, Affinity, Adsorption chromatography. Result analysis and interpretation. (62:05)
Day 17- Implicationsof HPLC and LCMS in Proteomics. Result analysis and interpretation. (65:33)
Day 18- Definition and Principle. Factors affecting electrophoresis and types of the gel matrix. Types of Gel-electrophoresis for proteins and their applications. (66:08)
Day 19- Detection, recovery, and characterization of proteins and their zymography (60:52)
Day 20- 2D-PAGE/Isoelectric focussing, Mass Spectroscopy – MALDI-TOF: Analysis and interpretation. Immunofluorescence. (66:23)
Day 21- Compilation of advanced proteomics, research prospects, and design. (60:34)

Day 13- Chromatography: Principle of distribution/partition coefficient. Absorption and Adsorption phenomenon, Retention factor (Rf and Rt concepts). Types: Planar and Columnar (non-pressure and pressure-induced systems).