Day 2 : Concept of E-S Complex, Binding Sites, Active Site,

Enzymology Certification Course

Day 1 : Introduction To Enzymes (Nomenclature and Classification), Activation Energy & Transition State Theory (82:10)
Day 2 : Concept of E-S Complex, Binding Sites, Active Site, Specificity, Apoenzyme, Holoenzyme (83:00)
Day 3 : Enzyme-Enzyme Interaction , Protein Ligand Binding (kinetics), Measurement Analysis of Binding Isotherm, Cooperativity, Hill and Scatchard plots (69:27)
Day 4 : Properties & Mechanism Of Enzyme Catalyzed Reaction, Two Substrate Reactions(random, ordered and ping pong mechanisms) (69:40)
Day 5 : Michaelis Menten Enzyme Kinetic and Plots : Lineweaver-Burk, Eadie-hofstee, Hanes-Woolf, Dixon’s (66:39)
Day 6 : Factors Affecting Enzyme Activity, Cofactors, Biocatalysts (58:02)
Day 7 : Enzyme regulation: Product Inhibition, Feedback Control, Covalent Modification, Allosteric Enzymes (75:57)
Day 8 : Enzyme Inhibition (Reversible and Irreversible) (69:45)
Day 9 : Zymogens, Isoenzymes, Multienzyme Complexes & Multifunctional Enzymes (with examples) (67:21)
Day 10 : Enzyme Preparation Techniques (Isolation, crystallization and purification of enzymes, enzyme preparation test of homogeneity, methods of enzyme analysis) (73:49)
Day 11 : Methods For Protein Sequencing & Analysis Of Secondary & Tertiary Structure of Enzymes, Protein Folding in vitro and in vivo (62:36)
Day 12 : Methods For Large Scale Production of Enzymes; Production of Enzymes in Heterologous Systems (63:06)
Day 13 : Immobilized Enzymes, Comparison With Soluble Enzymes, Methods Involved, Immobilized Enzyme Reactors, Applications (52:03)
Day 14 : Applied Enzymology (Fundamentals & Strategies of Enzymatic Assays, Enzyme Engineering, Abzymes, Ribozymes) (63:11)

Day 2 : Concept of E-S Complex, Binding Sites, Active Site, Specificity, Apoenzyme, Holoenzyme